The World’s First Dedicated
Microwave Peptide Cleavage System
• Full peptide cleavage in 30 minutes or less
• Microcleavage in as little as 2 minutes
• Improved peptide purity
• Increased peptide yield
• Use of less offensively smelling reagents
Peptide cleavage is one of the most important steps in peptide synthesis. If the cleavage fails to produce product or cleavage is incomplete, the results will be thrown off and the entire synthetic process may need to be repeated. Controlled and accurate cleavage is, therefore, critical to peptide synthesis success.
The Accent Peptide Cleavage System performs peptide cleavage quickly and easily to help get the answer you need in the timeframe you require.
Microcleavage in 2 Minutes
Microcleavage can be performed during peptide synthesis to accurately determine the progression of the synthesis by using LC/MS. Performing a microcleavage step is much more definitive than a Kaiser test and there is no better way to measure the peptide quality of an on-going synthesis. Unfortunately, a conventional microcleavage can take over an hour to obtain the results, wasting precious synthesis time in the process. In the Accent, microcleavage can be performed in as little as 2 minutes, allowing the rapid determination of the quality of the synthesis and resulting in very little time away from performing the synthesis itself.
Conditions: 20 mg resin, 2.5 mL TFA/TIS/H2O/DODT (92.5/2.5/2.5/2.5), 38 °C
Cleavage on the Accent can be performed at three different scales, depending on the amount of peptide required. The disposable fritted reaction vessel comes in three sizes:
• 4 mL vessel for microcleavage,
The fiber optic temperature probe provides in-situ measurement that controls the power output ensuring faster reactions, higher yields, and improved purity. When used with CEM’s patented single-mode technology, designed to automatically focus microwave energy with maximum efficiency, the Accent acts as the final stage in revolutionizing peptide synthesis.
• 20 mL vessel for 0.1 mmol cleavage, and
• 35 mL vessel for larger scale cleavage (up to 0.25 mmol).